1) GoTo settings >> Control Panel >> Java.
2)In Java >> View, add following text in "Runtime Parameters"
-Djava.compiler=NONE -Xnoagent -Xdebug -Xrunjdwp:transport=dt_socket,address=1045,server=y,suspend=y
3) Configure Remote Debugging in Eclipse. Give port as 1045(Or any port that you are using).

4) Start browser. Navigate to the page containing Applet.
5) As soon as Applet starts getting loaded, in eclipse it will stop at breakpoint & user can debug as normal java debugging.
6)Sometimes browser may get hanged or crashed, in such cases disconnect debugging from Eclipse & Click on page containing applet & then relaunch remote debugging in Eclipse.